Shruti Bhat PhD, MBA, Lean Six Sigma Black Belt
  • Home
  • About Shruti
  • Consulting
  • Workshops
  • Patents
  • Articles
    • Case Studies
    • Innovation Portfolio Development & Patents
    • Drugs-in-the- making
    • Culture Change & Business Transformation
    • Operational Excellence, Quality & Continuous Improvement
  • Books
  • Digital
  • Blog
  • Videos
  • Contact

Microbial contamination in lake and dye colors- Part 3 of 3

11/1/2013

0 Comments

 
Continued from Part 2 ...
​

Isolation and identification of bacteria-


The isolation was done by transferring the colonies from nutrient agar plated to sterile enrichment broth media. These included MacConkeys broth (for detection of Staph.aureus) and Cetrimide broth (detection of P.aeruoginosa). The specimen tubes were incubated for 48 hours at 37 + 1 oC in inverted position for
24 hours and 507 days.

Allocation of mannitol salt agar B30-

The colonies grown on Vogel –Johnson’s medium was streak plated on sterile mannitol –salt agar medium for confirmation regarding presence/ absence of Staph. aureus . Pure staphylococcus culture ATCC 12600 was simultaneously streaked on separate plates to serve as positive control.

Further study of culture on selective media-

The colonies grown on Vogel- Johnson’s medium and MacConkeys medium were tested for Gram reactions and motility characteristics.

Biochemical tests-

In order to further ensure that the organisms cultured on Vogel- Johnson’s and Mac Conkeys agar were probably E.coli, Staph. aureus or Salmonella species, the inoculums of the colonies from respective agar medium were employed in biochemical tests comprising of hydrolysis of starch, nitrate reduction, oxidase and catalase activity, liquefaction of gelatin, IMVic, Oxferm reaction etc. 
 
The contents of this article belong to extensive laboratory work done by me to assess microbial contamination on few brands of dye and lake colors available in the market.
​Follow Shruti on Twitter, Facebook, YouTube, LinkedIn
#MicrobialContamination #DyeColors  #LakeColors
0 Comments

Your comment will be posted after it is approved.


Leave a Reply.

    Shruti Bhat, global leader in business turnaround, operational excellence and continuous improvement

    New Book Released!

    top ten strategic decision-making tools for operational excellence

    Shruti's YouTube Channel ...

    Picture

    Blog Categories

    All
    3D Printing
    5S
    Agile
    Artificial Intelligence
    Automation
    Balanced Score Card
    Biotechnology
    Books
    Business Turnaround
    Case Studies
    Change Management
    Chemical Industry
    CMMI
    Continuous Improvement
    Design Thinking
    Digitalization
    Drug Delivery
    External News Links
    Food Industry
    Hall Of Fame
    Healthcare
    Hoshin Kanri
    HR Development
    Innovation
    ISO
    Just In Time
    Kaizen
    Leadership
    LEAN
    Lean Six Sigma
    Life Sciences
    Machine Learning
    Manufacturing
    Medical Devices
    Mistake Proofing
    Motivational Cards
    Nanotechnology
    Operations
    Packaging
    Patents
    Personal Products
    Process Improvement
    Product Development
    Productivity Increase
    QbD
    Quality Management
    Robotics
    Scrum
    Service Industry
    Six Sigma
    Strategy
    Supply Chain Logistics
    Telecom Industry
    TQM
    Videos
    Voice Of Customer
    Workshops

    Shruti's books...

    Picture
    shruti bhat, business process management, continuous improvement
    kaizen for pharmaceutcials, medical devices and biotech industry book by Dr Shruti Bhat
    Book on Continuous improvement tools by Dr Shruti Bhat
    kaizen for leaders, continuous process improvement tool to increase profit and organizational excellence by shruti bhat
    kaizen, shruti bhat, continuous improvement, quality, operations management
    Picture
    how to overcome challenges of creating effective teams
    how to lead a successful business transformation

    ​Micro-Learning
    ​Partner

    Picture

    Publishing Partner

    fast read books, business management books, health and wellness books, spiritual guidance books, best online book store

    Wellness
    ​Partner

    Picture
© Copyright 1992- 2022 Dr. Shruti Bhat ALL RIGHTS RESERVED.
See Terms and Conditions for details on this site usage.
SHRUTI BHAT, CONTACT
Click to connect.
Created by Macro2Micro Media